Optimized ultrasonic-assisted extraction of polysaccharides from <i>Cyclina sinensis</i> and evaluation of antioxidant activities in vitro

Abstract

The Box-Behnken design combined with response surface methodology was used to optimize ultrasonic-assisted extraction of polysaccharides from Cyclina sinensis. A high-extraction yield of polysaccharides, 34.63 ± 1.52 g/kg using an ultrasonic power of 500 W, liquid to material ratio of 22:1 (mL/g), with an extraction temperature of 77°C for 31 min was achieved. The polysaccharides exhibited quite a strong reducing property, the total antioxidant capacity, as well as excellent inhibitions of DPPH radical, hydroxyl radical, and superoxide radical. Meanwhile, it was found that the bioactivities appeared to be dose-dependent on polysaccharides.