Culture conditions promoting dispersed growth and biphasic production of actinorhodin in shaken cultures of<i>Streptomyces coelicolor</i>A3(2)

Abstract

Media and culture conditions were developed for experiments on the physiology of secondary metabolism in Streptomyces coelicolor A3(2). Well dispersed mycelial growth was obtained in a buffered starch-glutamate-salts medium; a high (5%) starch concentration and addition of glass beads aided dispersal. Under the conditions developed, production of actinorhodin was suppressed during trophophase growth and began abruptly near the growth maximum.