The quantification of gene expression in an animal model of brain ischaemia using TaqMan™ real-time RT-PCR

Real time quantitative PCR.

Chris Heid, Junko Stevens, Kenneth J. Livak et al. · Genome Research · 1996 · 6.2K citations · Full text

Real-time Monitoring, Engineering, Measurement +17

Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Pamela M. Holland, Richard D. Abramson, R. R. Watson et al. · Proceedings of the National Academy of Sciences · 1991 · 2.7K citations · Full text

Undegraded Probe, Polymerase Chain Reaction, Engineering +15

Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

Russell Higuchi, Carita Fockler, Gavin Dollinger et al. · Nature Biotechnology · 1993 · 2.2K citations

Polymerase Chain Reaction, Engineering, Medicine +11

A novel method for real time quantitative RT-PCR.

U E Gibson, Chris Heid, P. Mickey Williams · Genome Research · 1996 · 2.1K citations · Full text

Real-time Monitoring, Engineering, Measurement +19

Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

Kenneth J. Livak, Samantha Flood, J Marmaro et al. · Genome Research · 1995 · 1.6K citations · Full text