Cellular mechanisms of glucocorticoid immunosuppression in salmon

Abstract

Limiting dilution analysis was employed to determine the effect of cortisol and conditioned media on precursor frequency and clone size of antibody‐producing cells sensitive to trinitrophenylated‐lipopolysaccharide (TNP‐LPS). This analysis demonstrated that the restoration of the cortisol‐suppressed antibody response by conditioned media occurs through the activation of previously inhibited B cell precursors to that antigen, and not by the recruitment of other antigen‐insensitive B cell precursors.