Biological and Pharmaceutical Bulletin · 2010 · 11 citations · 5 references
AgricultureBiotechnologyGeneticsHerbicide ResistancePlant BiotechnologyPlant GeneticsMolecular DiagnosisMolecular Markers In PlantsPolymerase Chain ReactionResistance DiagnosisResistance Gene DiscoveryResistance MonitoringAcetolactate Synthase Gene AmplificationHerbicide Resistance ScreeningPolymerase Chain Reaction IdentificationPolymerase Chain Reaction Product DetectionPolymerase Chain Reaction ScreeningPolymerase Chain Reaction-based ScreeningReal-time Detection AssaysReal-time Polymerase Chain Reaction Assays
During the fall of 2009, a trace of unauthorized genetically modified (GM) flax (Linum usitatissimum L.) line, CDC Triffid, which is resistant to sulfonylurea herbicides, was detected in many countries including Japan. A method to reliably identify the CDC Triffid line was urgently required. We developed a novel construct-specific real-time polymerase chain reaction (PCR) method to identify the mutant acetolactate synthase gene in the CDC Triffid line. We confirmed that the method can detect 0.001% GM flax in DNA mixing solution. The study shows that the developed method is specific, sensitive and reliable way to monitor a trace of CDC Triffid.
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