The Journal of Clinical Endocrinology & Metabolism · 1955 · 10 citations · 6 references
Life SciencesBiochemistryEndocrinologyHormone BiochemistryReproductive EndocrinologySteroid BiochemistryEndocrine TestingGonadal HormonesHormonal EvaluationHormone MetabolismSteroid ConjugationSteroid Hormone BiochemistryCleavageConcentrationEstradiol AssayEstradiol MeasurementEstrogen ConjugationSex Steroid MeasurementUrinary Hormone MeasurementUrinary Steroid Analysis
THE results reported in this communication were obtained in the course of a study requiring the determination of estrogens and pregnanediol in a large number of urine samples which had been stored for a long period of time. The determination of estrogens in human pregnancy urine has to to be preceded by the complete cleavage of all estrogen conjugates in such urines, and this should be accomplished without any destruction of the liberated estrogenic steroids if these determinations are to be truly quantitative. A method satisfying these requirements has not been described as yet (1). Therefore an attempt has been made to ascertain the conditions for optimal recovery of estrogens from their conjugates; and the subsequent extraction and concentration procedure has been simplified to the greatest extent consistent with a quantitative recovery of the deconjugated estrogens. The estrogens were determined by a modification of the Kober test (2) described by Allen (3), and the and the conditions for maximal efficiency of this test were ascertained.
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