Rapid Communications in Mass Spectrometry · 1998 · 40 citations · 0 references
Life SciencesPharmaceutical AnalysisPharmacologyAnalytical ChemistryChromatographic AnalysisDrug MetabolismLiquid ChromatographyMass SpectrometryMass Spectrometry-based MetabolomicsMetabolite IdentificationMetabolite ProfilingUntargeted MetabolomicsChromatographic Drug AnalysisDrug Metabolite ProfilingLiquid Chromatography-mass SpectrometryLiquid Chromatography-mass Spectrometry AnalysisLiquid Chromatography-mass Spectrometry Method DevelopmentMass Spectrometry-based Metabolite ProfilingTandem Mass SpectrometryTandem Mass Spectrometry Analysis
A rapid and systematic strategy for the identification of drug metabolites in biological matrices based on liquid chromatography-tandem mass spectrometry (LC/MS/MS) techniques was utilized for the identification of drug metabolites of the HIV protease inhibitor Indinavir. This strategy integrates intelligent realtime mass spectrometry with HPLC detection and a predictive strategy for detecting metabolites arising from common biotransformations, to rapidly elucidate structures of drug metabolites. Structures of metabolites generated from in vitro incubation mixtures of Indinavir were characterized from a single chromatographic analysis using the automated LC/MS/MS methodology, thus reducing data acquisition time and improving efficiency.