Inhibition by Mannose of In Vitro Colonization of Chicken Small Intestine by Salmonella typhimurium

Abstract

The in vitro adherence of [3H]thymidine-labeled Salmonella typhimurium isolates to the small intestine of one-day-old chickens was investigated. Bacteria were screened for mannose sensitivity and mannose-resistance binding properties. Type 1 fimbriae positive strains adhered significantly better than Type 2 fimbriae-negative strains. Adherence was significantly (P less than .05) inhibited by D-mannose, methyl-alpha-D-mannoside, arabinose, and galactose. Adherence was both time and temperature dependent. These findings suggest that the small intestine of the chicken has receptors for bacteria with Type 1 fimbriae. The function of the receptors is dependent on a mannose moiety. Bacteria adhered better to fresh intestine cells than to cells held overnight at 4 C. Thus, adherence was dependent upon a metabolically active host cell. The in vitro adherence assay may further be used to study the interaction of bacteria with chicken enterocytes.