Concepedia

An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH

P. Early, Huarong Huang, Mark M. Davis, Kathryn Calame, L Hood

Cell · 1980 · 866 citations · 21 references

DOI

Concepts

References

21

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975

+15

32.8K citations

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

W. David Benton, Ronald W. Davis · Science · 1977

+22

4.7K citations

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Günter Blobel, B Dobberstein · The Journal of Cell Biology · 1975

+18

3K citations

The isolation of structural genes from libraries of eucaryotic DNA

Tom Maniatis, Ross C. Hardison, Elizabeth Lacy et al. · Cell · 1978

+11

1.9K citations

The use of thin acrylamide gels for DNA sequencing

Frederick Sanger, Alan Coulson · FEBS Letters · 1978

+5

1.4K citations