Science · 2001 · 195 citations · 20 references
Life SciencesBiochemistryMolecular BiologyDna RepairEnzymologyNucleic Acid BiochemistryBase Excision RepairDna Double-strand Break RepairDna LigaseDna Polymerase FunctionDouble-strand Break RepairNucleotide Excision RepairApyrimidinic EndonucleasesDna Ligase FunctionDna Polymerase CatalysisDna Polymerase IEnzymatic Catalysis MechanismsHuman Dna Ligase ILyase CatalysisSpecialized Dna Polymerases
DNA polymerase iota (pol iota) is one of several recently discovered DNA polymerases in mammalian cells whose function is unknown. We report here that human pol iota has an intrinsic 5'-deoxyribose phosphate (dRP) lyase activity. In reactions reconstituted with uracil-DNA glycosylase (UDG), apurinic/apyrimidinic (AP) endonuclease and DNA ligase I, pol iota can use its dRP lyase and polymerase activities to repair G*U and A*U pairs in DNA. These data and three distinct catalytic properties of pol iota implicate it in specialized forms of base excision repair (BER).
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Eukaryotic polymerases ι and ζ act sequentially to bypass DNA lesions
Robert E. Johnson, M. Todd Washington, Lajos Haracska et al. · Nature · 2000 · 627 citations
Mammalian Abasic Site Base Excision Repair
Deepak Srivastava, Brian J. Vande Berg, Rajendra Prasad et al. · Journal of Biological Chemistry · 1998 · 379 citations · Full text