Isolation of a novel cDNA encoding a zinc-finger protein that binds to two sites within the c-myc promoter

Abstract

The ME1a1 and ME1a2 elements are cis-acting DNA sequences that exist at positions -46 and -85, respectively, within the P2 promoter of the c-myc gene. These elements are required for optimal transcription initiation from P2. The proteins that bind to these elements are identical or very similar. Here we have isolated a cDNA clone that encodes the carboxy-terminal 494 amino acids of the human ME1a1/ME1a2 factor. This factor is referred to as "ZF87" (zinc-finger protein, 87 kilodaltons). ZF87 specifically binds the ME1a1 element with higher affinity than the ME1a2 element. Western blot analysis indicates that the full-length protein has a relative molecular mass of approximately 87,000 daltons, and Northern blot analysis shows that it is encoded by a 5-kb transcript. ZF87 contains six zinc-finger domains, of the Cys2-His2 type, at the carboxy terminus of the protein. The protein also contains extended tracts of polyalanine.