Preparations for the use of<i>Candida rugosa</i>lipase in non-conventional solvents

Abstract

Enzymatic reactions in organic media offer new possibilities for producing useful chemicals. The use of enzymes in both macro- and micro-aqueous systems has been investigated intensively in the last two decades and numerous studies have been done to improve their activity in organic solvents. Lipases are the most investigated class of enzymes studied in organic media and that from Candida rugosa has been explored the most due to its broad specificity, abundance and cost-effectiveness. The present review discusses the technologies employed in obtaining functional C. rugosa lipase in organic solvents and their use in various reactions. A brief description of structural insights of the enzyme in organic solvents is also provided.