Applied Spectroscopy · 2011 · 21 citations · 29 references
Natural SciencesBiochemistryChemistrySpectroscopic AnalysisSpectroscopyStructural BiologyBiophysical SpectroscopyInfrared SpectroscopyMolecular SpectroscopyOptical SpectroscopySpectroscopy Of BiomoleculesTerahertz SpectroscopyFar-infrared SpectroscopyFourier TransformFourier Transform Infrared SpectroscopyInfrared Absorption SpectraInfrared Absorption SpectroscopyInfrared SpectraMolecular Vibrational ModesVibrational Modes
Terahertz time-domain spectroscopy (THz-TDS) and Fourier transform infrared (FT-IR) spectroscopy were used to generate far-infrared and low-frequency spectral measurements of monomeric lysozyme and lysozyme fibrils. The formation of lysozyme fibrils was verified by the Thioflavin T assay and transmission electron microscopy (TEM). It was evident in the FT-IR spectra that between 150 and 350 cm(-1) the two spectra diverge, with the lysozyme fibrils showing higher absorbance intensity than the monomeric form. The broad absorption phenomenon is likely due to light scattered from the fibrillar architecture of lysozyme fibrils as supported by simulation of Rayleigh light scattering. The lack of discrete phonon-like peaks suggest that far-infrared spectroscopy cannot detect vibrational modes between the highly ordered hydrogen-bonded beta-pleated sheets of the lysozyme subunit.
29
Mechanism of thioflavin T binding to amyloid fibrils
Chris L. Coleman, Cristian Ionescu‐Zanetti, Sue A. Carter et al. · Journal of Structural Biology · 2005 · 823 citations