Scanning coherent anti-Stokes Raman microscope

Abstract

We have constructed a spatially scanning coherent anti-Stokes Raman spectroscopic (CARS) apparatus that allows us to image the distribution of distinct chemical species in a microscopic sample region. Images of onion-skin cells have been obtained by using the CARS signal produced by the 2450-cm(-l) band of deuterated water. Future applications will be discussed.