Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability

Abstract

In this study, the Reactive Oxygen Species (ROS) levels in Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) with different cell viability were measured by ROS probe fluorescence. It is found that the change of the ROS level with time (viability, decline) can be described by a Rational Function, whose integration can calculate the relative yield variations of ROS in different durations. The relative intensity variations of the characteristic Raman peak at 1342, 877, and 744 cm−1 were observed using Raman micro-spectroscopy. The results show that the relative yield variation of ROS and the relative intensity variations of the three Raman peaks can be described by a same model function (with different fitting parameter values). And the correlations between the relative intensity variations of the three Raman peaks and the relative yield variation of ROS were estimated by correlation coefficient method. It is found that they have perfect linear correlation coefficients of 0.980, 0.985, and 0.973, respectively. The analysis demonstrates that the intracellular excessive ROS can lead to the almost linear variation of the relative Raman peak intensity in hUC-MSCs with different viability.