Influence of the Kind of Alcoholic Modifier on Chiral Separation on a Pirkle Model Chiral Column

Abstract

Abstract Separation of the enantiomers of two series of seven organic phosphonates on π acidic N-(3,5-dinitrobenoyl) leucine chiral stationary phase is described. Enantiomer resolution is highly dependent on the mobile phase composition and the structure of the enantiomers. One of seven enantiomers can be separated using hexane-2-propanol mobile phase, other enantiomers can be partly separated only. The six out of seven enantiomers are separated and the retention times of all enantiomers are significantly decreased using ethanol instead of 2-propanol. The separation of all enantiomers is hardly affected by varying column temperature (10–40°C).