Effect of Oleic Acid on the Oxidation and Gluconeogenesis from [1‐¹⁴C]Pyruvate in the Perfused Rat Liver

Abstract

Gluconeogenesis from [1‐ 14 C]pyruvate in the perfused liver from fasted rats was stimulated by oleic acid as indicated by an increase in medium glucose, liver glycogen and specific activity of medium [ 14 C]glucose. A balance of carbon from pyruvate revealed that in the presence of oleic acid 2 moles of pyruvate were utilized for each mole of glucose synthesized in contrast to a ratio 4:1 without fatty acid. From this and 14 CO 2 data it is concluded that oleic acid inhibited pyruvate oxidation markedly. Glucagon increased glucose formation from pyruvate but not from glycerol.