Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants

TLDR

The authors engineered 43 Gateway binary vectors (pGWBs) incorporating 15 diverse reporters and tags, optional 2×35S‑Ω promoters, and selectable kanamycin or hygromycin markers to facilitate versatile plant transformation. The compact, high‑copy pGWBs simplify plasmid preparation and sequencing, positioning them as a powerful resource for plant transgenic research.

Abstract

We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6×His, FLAG, 3×HA, 4×Myc, 10×Myc, GST, T7, and TAP, were employed. Some vectors carry the 2×35S-Ω promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.