Sequential, Structural, and Phylogenetic Study of BRCT Module in Plants

Abstract

The BRCT (Breast Cancer Carboxyl Terminus) domain is widely distributed in proteins involved in DNA metabolism and cell cycle regulation. In most of the representative members of the BRCT family, this domain is usually comprising of about 90-100 amino acid residues and generally present as single motif or in tandem repeats. Although the members of BRCT family share little sequence similarity, structural studies have demonstrated a relatively conserved structure of two or three alpha-helices surrounding the central beta-sheets. This report illustrates an in silico analysis with the aim of understanding the sequential, structural, and phylogenetic features of BRCT domain in higher plant genome. Based on database searches 25 BRCT domain containing proteins were identified and many of them were found to be involved in multiple DNA damage repair pathways. We have further combined the homology modeling in order to address the structure-function relations of BRCT domain in connection with DNA damage repair mechanism in plants.