Solid Phase Enzyme Immunoassays for the Quantification of Serum Amyloid P (SAP) and Complement Component 3 (C3) Proteins in Acute-Phase Mouse Sera

Abstract

Simple, reliable and sensitive enzyme immunoassays have been developed for the quantification of the mouse acute-phase SAP and C3 proteins. The ELISA systems were validated using sera from mice injected with S. dysenteriae endotoxin, and detected 500 pg protein/ml. The assays use 96-well microtitre plates permitting rapid processing of a large number of samples.