The Journal of Cell Biology · 1995 · 509 citations · 49 references
Life SciencesBiochemistryMolecular BiologyGene ExpressionNucleic Acid BiochemistryStructural BiologyNuclear-cytoplasmic TransportProtein LocalizationProtein StructureProtein-nucleic Acid InteractionsRna LocalizationRna-binding ProteinsAmino AcidsMessenger Rna Binding ProteinsNuclear Localization SignalsNucleotide Binding DomainsNucleotide-binding MotifRna Localization ElementsRna-binding DomainsRna-binding Motif
hnRNP A1 is a highly abundant nuclear RNA‑binding protein with two RNA‑binding domains and a glycine‑rich tail, yet it lacks a conventional nuclear localization signal. A ~40‑residue C‑terminal segment, M9, was identified as a novel nuclear localization domain that can redirect cytoplasmic proteins to the nucleus and is not dependent on the protein’s RNA‑binding motifs.
The heterogeneous nuclear RNP (hnRNP) A1 protein is one of the major pre-mRNA/mRNA binding proteins in eukaryotic cells and one of the most abundant proteins in the nucleus. It is localized to the nucleoplasm and it also shuttles between the nucleus and the cytoplasm. The amino acid sequence of A1 contains two RNP motif RNA-binding domains (RBDs) at the amino terminus and a glycine-rich domain at the carboxyl terminus. This configuration, designated 2x RBD-Gly, is representative of perhaps the largest family of hnRNP proteins. Unlike most nuclear proteins characterized so far, A1 (and most 2x RBD-Gly proteins) does not contain a recognizable nuclear localization signal (NLS). We have found that a segment of ca. 40 amino acids near the carboxyl end of the protein (designated M9) is necessary and sufficient for nuclear localization; attaching this segment to the bacterial protein beta-galactosidase or to pyruvate kinase completely localized these otherwise cytoplasmic proteins to the nucleus. The RBDs and another RNA binding motif found in the glycine-rich domain, the RGG box, are not required for A1 nuclear localization. M9 is a novel type of nuclear localization domain as it does not contain sequences similar to classical basic-type NLS. Interestingly, sequences similar to M9 are found in other nuclear RNA-binding proteins including hnRNP A2.
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A short amino acid sequence able to specify nuclear location
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