Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

TLDR

The study aims to develop reagents for quantifying p62c‑myc in cells. Monoclonal antibodies against p62c‑myc were produced and employed in an immunoblotting assay. Six monoclonal antibodies were isolated; five precipitate p62c‑myc from human cells, three also recognize mouse c‑myc, none bind chicken p110gag‑myc, and all detect immunoblotted p62c‑myc.

Abstract

Six monoclonal antibodies have been isolated from mice immunized with synthetic peptide immunogens whose sequences are derived from that of the human c-myc gene product. Five of these antibodies precipitate p62c-myc from human cells, and three of these five also recognize the mouse c-myc gene product. None of the antibodies sees the chicken p110gag-myc protein. All six antibodies recognize immunoblotted p62c-myc. These reagents also provide the basis for an immunoblotting assay by which to quantitate p62c-myc in cells.