Enzyme-Catalyzed Reactions of the Carcinogen <i>N</i> -Hydroxy-2-fluorenylacetamide with Nucleic Acid

Abstract

A protein fraction obtained by gel filtration of a 105,000g supernatant of rat liver catalyzes three reactions of the hepatocarcinogen N-hydroxy-2-fluorenylacetamide with nucleic acid. Cofactor requirements and isotopic studies suggest that the reactive intermediates involved may be N-2-fluorenylhydroxylamine, and phosphate and sulfate esters of N-hydroxy-2-fluorenylacetamide.