Journal of Liquid Chromatography & Related Technologies · 2001 · 16 citations · 5 references
PharmacyMedicinal ChemistryPharmaceutical AnalysisChromatographic AnalysisDrug DiscoverySeparation ScienceAnalytical ChromatographyAromatic PlantsChromatographyGradient ElutionLiquid ChromatographyMetabolite ExtractionActive Compound IsolationBioactive Compound IsolationChromatographic Analysis Of PhenolicsHerbal Drug AnalysisMedicinal HerbsPhenolic Compound ExtractionPhenolic Compound SeparationTraditional Chinese Medicine
Abstract Analytical and preparative high-speed countercurrent chromatography was successfully used for the separation of hydroxyanthraquinones from traditional Chinese medicine such as Rheum officinale Baill (Dahuang) and Polygonum cuspidatum Sieb. Et Zucc (Huzhang) using pH-modulated stepwise elution. Four major components including chrysophanol, emodin, physcion, and aloe-emodin were isolated each at over 98% purity. ACKNOWLEDGMENTS Financial support from Beijing Academy of Science & Technology and Beijing Commission of Science & Technology is gratefully acknowledged. We also thank senior engineer Xining Li for the excellent technical assistance with the research work.
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