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Near-field optical microscopy in liquids
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1995
Year
EngineeringMicroscopyBiomedical EngineeringMicroscopy MethodOptical DiagnosticsDynamic Mode AfmLight MicroscopyBiophysicsMaterials ScienceMedicineImagingNoncontact Mode AfmBiophotonicsOptical SensorsFluorescence MicroscopyBiomedical DiagnosticsScanning Probe MicroscopyMaterials CharacterizationScanning Force MicroscopyProbe CantileverNear-field Optical MicroscopyAcoustic Microscopy
The scanning near-field optical microscopy imaging of specimens in liquid and of cultured cells in aqueous solutions is reported. A scanning near-field optical/atomic-force microscope (SNOM-AFM) was developed, in which the scanning of an optical-fiber probe cantilever over the specimen was controlled by noncontact mode AFM (dynamic mode AFM). This imaging mode reduces damage to the probe and soft specimens. The resonant frequency of the probe cantilever decreased 20% to ≊14 kHz and the Q factor decreased by a factor of 8 to ≊30 in water, compared with these values in air, which was sufficient to perform SNOM-AFM imaging in liquid.