Preparation of Anti-lipolytic Substance from Panax ginseng

Abstract

It was found that water extract of Panax ginseng strongly inhibited adrenaline-induced lipolysis in isolated fat cells of rat epididymal adipose tissue. Anti-lipolytic action of the water extract was easily inactivated by treatment with pronase, suggesting that the active principle might be a protein or a peptide. The water extract was dialyzed against distilled water. The outer dialysate was treated with DEAE-cellulose and the eluate was subjected to gel filtration on Sephadex G-50 column, Avicel cellulose column chromatography and phosphocellulose column chromatography, successively. With these procedures, a peptide (P II), which had anti-lipolytic activity, was isolated in a purified form. Key Word Index Panax ginseng - Araliaceae - Anti-lipolytic Substance - Fat Cells - Adrenaline-induced Lipolysis.