Growth and Organogenesis in Tissue Cultures of <i>Allium cepa</i> var. <i>proliferum</i>

Abstract

Abstract Callus isolated from aerial bulbs of Allium cepa var. proliferum was grown in agar and liquid cultures on a synthetic medium containing 5 × 10 −6 M 2,4‐D. Root formation occurred in the absence of 2,4‐D and was highly stimulated by 5 × 10 −6 M NAA. Cytokinin was not necessary for growth and organ formation but slightly stimulated the formation of leafy buds. Combinations of NAA or IAA and cytokinin stimulated growth and root formation to a greater extent than anyone of these substances added alone. Pieces of callus in liquid culture developed roots in one week in root‐inducing medium, but bud or embryo formation was not observed in liquid cultures.