Identification of brassinolide and castasterone in buckwheat (Fagopyrum esculentum Moench) pollen.

Abstract

From the pollen of buckwheat (Fagopymm esculentum Moench), the bioactive substances in a rice lamina inclination test were extracted and partially purified by several chromatographic methods. They were derivatized with 9-phenanthreneboronic acid and dansylaminophenylboronic acid, and then analyzed by reversed-phase high-performance liquid chromatography with fluorimetric detection. The active compounds were identified as brassinolide and castasterone by co-chromatography using authentic samples. In addition, these brassinosteroids were rigorously identified as their bismethaneboronate derivatives by the gas chromatography/high resolution-selected ion monitoring method. These data clearly established the presence of brassinolide and castasterone in buckwheat pollen.