FEMS Microbiology Letters · 2004 · 67 citations · 37 references
Life SciencesMicrobiologyMicrobial GeneticsMicrobial MetabolismNatural Product BiosynthesisAntibiotic ProductionMicrobial Metabolite ProductionMicrobial Natural Product BiosynthesisAntimicrobial MetabolitesBacterial Natural Product BiosynthesisInducible OperonsProduction Of Antimicrobial CompoundsPseudomonas SpeciesRepressible OperonsTwo-component Regulatory Systems
The biosynthesis of antimicrobial metabolites is controlled by the GacS/GacA two-component regulatory system in Pseudomonas species. The production of phenazine-1-carboxylic acid and pyoluteorin is differentially regulated by GacA in Pseudomonas sp. M18. Pyoluteorin was reduced to nondetectable level in culture of the gacA insertional mutant strain M18G grown in King's medium B broth, whereas phenazine-1-carboxylic acid production was increased 30-fold over that of the wild-type strain. Production of both antibiotics was restored to wild-type levels after complementation in trans with the wild-type gacA gene. Expression of the translational fusions phzA'-'lacZ and pltA'-'lacZ confirmed the effect of GacA on both biosynthetic operons.
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Molecular cloning: A laboratory manual
Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations
Two simple media for the demonstration of pyocyanin and fluorescin.
Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria
N. T. Keen, Stanley Tamaki, Daisuke Kobayashi et al. · Gene · 1988 · 1.5K citations