Photochemistry and Photobiology · 1978 · 109 citations · 33 references
Life SciencesMicrobiologyMolecular BiologyDna RepairMicrobial GeneticsRadiation BiophysicsBiological Effects Of RadiationDna CrosslinkingDna DamageGenotoxicity MechanismsRadiation SensitivityUltraviolet Radiation BiologyEscherichia ColiIrradiation EffectsMicrobial Adaptation To Ultraviolet RadiationPsoralen-induced Dna CrosslinksUltraviolet Light TreatmentUltraviolet-induced CrosslinksUltraviolet-induced Dna DamageUv-induced Dna Lesions
Abstract— On treatment with 8‐methoxypsoralen plus near UV light, an excisionless ( uvrB ‐ ) strain of Escherichia coli showed about 3– and 10 times higher sensitivities to killing and mutation, respectively, than its parental strain. On re‐irradiation with near UV in the absence of unbound psoralen, the uvrB ‐ strain pretreated with psoralen plus near UV showed a decrease in both survival and mutation. After treatment with psoralen plus near UV, re‐irradiation of T7 DNA in the absence of unbound psoralen caused an increase in the cross‐linked fraction with an equivalent decrease in the non‐cross‐linked fraction. From these and previous results, we conclude that monoadducts produced by treatment with psoralen plus near UV are converted to cross‐links by further irradiation and that, in E. coli , monoadducts are responsible for the mutation induced by psoralen‐plus‐light whereas cross‐links are the major cause of its lethal action.
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PHOTOREACTIONS AT 3655Å BETWEEN PYRIMIDINE BASES AND SKIN‐PHOTOSENSITIZING FUROCOUMARINS
L. Musajo, F. Bordin, G. Caporale et al. · Photochemistry and Photobiology · 1967 · 221 citations
Medicine, Biochemistry, Metabolism +10