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Using E. coli-based cell-free protein synthesis to evaluate the kinetic performance of an orthogonal tRNA and aminoacyl-tRNA synthetase pair

BiosynthesisEngineeringBiochemistryKinetic PerformanceNatural SciencesProtein BiosynthesisBiotechnologyMolecular BiologySynthetic BiologyProtein EngineeringAminoacyl-trna Synthetase PairStructure-function Enzyme KineticsOrthogonal TrnaProtein Synthesis