Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text

Life Sciences, Biotechnology, Genetics +17

Transformation of intact yeast cells treated with alkali cations

Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983 · 7.7K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +10

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +15

[1] Production of single-stranded plasmid DNA

Jeffrey Vieira, Joachim Messing · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 2.6K citations

Life Sciences, Biotechnology, Molecular Biology +12