Functional redundancy and structural polymorphism in the large subunit of RNA polymerase II

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA

Ellson Y. Chen, Peter H. Seeburg · DNA · 1985 · 2.5K citations

Life Sciences, Microbiology, Molecular Biology +14

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink · Molecular and General Genetics MGG · 1984 · 2.4K citations

Life Sciences, Genetics, Molecular Biology +8

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Jef D. Boeke, Joshua Trueheart, Georges Natsoulis et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 1.4K citations

Life Sciences, Genetics, Molecular Genetics +7