Functional dissection of the yeast Cyc8–Tupl transcriptional co-repressor complex

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text

Life Sciences, Biotechnology, Genetics +17

A novel genetic system to detect protein–protein interactions

Stanley Fields, Ok-Kyu Song · Nature · 1989 · 6.1K citations

Life Sciences, Genetics, Molecular Biology +13

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Life Sciences, Biochemistry, Molecular Biology +12

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

R. Daniel Gietz, Akio Sugino · Gene · 1988 · 2.9K citations

Life Sciences, Genetics, Microbiology +13

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

Jeffrey Field, Jun‐ichi Nikawa, Daniel Broek et al. · Molecular and Cellular Biology · 1988 · 961 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +17