High-speed spectral imager for imaging transient fluorescence phenomena

Abstract

We describe fluorescence spectral imaging results with the microscope computed-tomography imaging spectrometer (muCTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, muCTIS can be used to image dynamic phenomena. The results presented consist of proof-of-concept imaging results with static targets composed of 6-mum fluorescing microspheres. Image data were collected with integration times of 16 ms, comparable with video-frame-rate integration times. Conversion of raw data acquired by the muCTIS to spatial and spectral data requires postprocessing. The emission spectra were sampled at 10-nm intervals between 420 and 710 nm. The smallest spatial sampling interval presented is 1.7 mum.