Coimmobilization of Acetylcholinesterase and Choline Oxidase on New Nanohybrid Membranes Obtained by Sol Gel Technology

Abstract

ABSTRACTIn this study, a multi-enzyme optical biosensor was constructed. Therefore, the first step was synthesis of different kinds of SiO2 hybrid membranes suitable for optical biosensors using sol-gel techniques. The aim of this study was to perform separate covalent immobilization and coimmobilization of the enzymes acetylcholinesterase and choline oxidase and to determine the catalytic properties of the new biocatalyst procedures on new nanohybrid membranes. The catalytic properties of the two enzymes were studied separately and simultaneously. Also, determination of the most suitable membrane for its subsequent application in the optical biosensors is important. This multienzyme membrane will be applied in the design of a fiber optic biosensor for pesticide detection.Keywords: optical biosensorhybrid membranescholine oxidaseacetylcholinesterase