Oncology · 2009 · 23 citations · 0 references
Life SciencesCell BiologyOncologyCancer GeneticsMolecular OncologyTumor ProgressionAneuploidyCancer CytogeneticsMalignant NeoplasmsMalignant TumorsMelanocytic TumorsTumor GradingCancer AneuploidyCell Cycle AnalysisFlow Cytometric AnalysisMalignant Melanocytic TumorsMelanocytic Tumor PrognosisMelanoma DiagnosisNeoplastic CytogeneticsTumor Thickness Prognosis
DNA flow cytometry was carried out on 804 primary melanomas. The data were analyzed with a follow-up of 24-96 months. 57% of the cases were diploid, 32% had one abnormal cell population, and 11% were multiclonal. In 8% of the aneuploid tumors there were cell lines in the hypertetraploid range. A reliable S phase determination was possible in 524 cases. Among these 11% had an S phase exceeding 15%. Using an increased tumor thickness, relapse rate and mortality as criteria of tumor progression, aneuploidy and multiclonality, the occurrence of hypertetraploid cell lines and a high S phase (greater than 15%) proved to be correlated with a poor prognosis.