MEASUREMENT OF SMALL QUANTITIES OF INSULIN-LIKE ACTIVITY USING RAT ADIPOSE TISSUE. II. EVALUATION OF PERFORMANCE*

Abstract

In the preceding paper (1), a biological assay method was proposed which utilized the increased production of CO2 from glucose in response to minute quantities of insulin by epididymal adipose tissue of rats. Since the quality of an assay method is best revealed by its performance over a period of time, the present report will present the results obtained during 25 months when this assay procedure was applied to insulin solutions and was tested on more complex material such as human blood serum. The quantitative evaluation of the data was carried out to a large extent independently from the actual performance of the assays, although consultations between the statistical and the biological teams occurred at irregular intervals of a few months.