Differentiation between <i>Haemophilus paraphrophilus</i>, <i>H. aphrophilus</i>, <i>H. influenzae</i>, <i>Actinobacillus actinomycetemcomitans</i>, <i>Pasteurella multocida</i>, <i>P. haemolytica</i>, and <i>P. ureae</i> by high resolution two‐dimensional protein electrophoresis

Abstract

Abstract The cellular proteins of Haemophilus paraphrophilus (ATCC 29242, ATCC 29241 T , ATCC 29240, HK 477, HK 319, HK 159), H. aphrophilus (ATCC 33389 T ), H. influenzae (ATCC 31441), Actinobacillus actinomycetemcomitans (ATCC 33384 T ), Pasteurella multocida (NCTC 10322 T ), P. haemolytica (9380 T ), and P. ureae (NCTC 10219 T ) were analyzed by high resolution two‐dimensional protein electrophoresis and silver staining. The electrophoretic protein patterns of Haemophilus , Actinobacillus and Pasteurella were quite distinct. Also the patterns of P. multocida, P. haemolytica and P. ureae differed markedly. Except for H. paraphrophilus ATCC 29242, it was difficult to distinguish H. paraphrophilus from H. aphrophilus strains. Excluding ATCC 29242, H. paraphrophilus strains were quite similar. A. actinomycetemcomitans could easily be distinguished from all species tested, including the related H. aphrophilus, H. paraphrophilus, P. haemolytica , and P. ureae . High resolution two‐dimensional protein electrophoresis is likely to contribute to a more unifying concept of bacterial species. Among the organisms examined only H. aphrophilus and H. paraphrophilus seem to requires revision of their current taxonomic positions.