Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria

Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

Gerard Muyzer, Ellen C. de Waal, André G. Uitterlinden · Applied and Environmental Microbiology · 1993 · 11.5K citations · Full text

Life Sciences, Genetics, Microbiology +16

The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations

Jan-henrich Rotthauwe, K.-P. Witzel, Werner Liesack · Applied and Environmental Microbiology · 1997 · 2.9K citations · Full text

Life Sciences, Microbiology, Molecular Biology +14

Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

Val C. Sheffield, David R. Cox, Leonard S. Lerman et al. · Proceedings of the National Academy of Sciences · 1989 · 1.3K citations · Full text

Life Sciences, Genetics, Molecular Biology +14

Phylogeny of All Recognized Species of Ammonia Oxidizers Based on Comparative 16S rRNA and <i>amoA</i> Sequence Analysis: Implications for Molecular Diversity Surveys

Ulrike Purkhold, Andreas Pommerening‐Röser, Stefan Juretschko et al. · Applied and Environmental Microbiology · 2000 · 1.1K citations · Full text

Life Sciences, Biology, Microbiology +17

[31] Detection and localization of single base changes by denaturing gradient gel electrophoresis

R Myers, Tom Maniatis, Leonard S. Lerman · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 936 citations

Life Sciences, Biochemistry, Molecular Biology +10