Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI‐D) resistance by primer‐introduced restriction enzyme analysis in scytalone dehydratase gene of <i>Magnaporthe grisea</i>

Abstract

Abstract We have established a simple diagnosis method for rice blast fungus resistant to MBI‐D. This involves the preparation of PCR templates directly from the lesions in combination with primer‐introduced restriction enzyme analysis PCR (PIRA‐PCR). Copyright © 2003 Society of Chemical Industry