Microwave miniprep of total genomic DNA from fungi, plants, protists and animals for PCR.

Abstract

A rapid procedure for the isolation of total DNA from various eukaryotic organisms is described. This method uses a common microwave oven for the alteration of cellular walls and membranes. The procedure is rapid and can be completed in as little as 15 minutes. The method has been used to isolate DNA from reptiles, plants, slime molds and other protists, and this DNA has been used for successful amplification of ribosomal genes from these organisms. The procedure is a time-saving and cost-effective process that helps to eliminate several sources of contamination that can be found in other DNA isolation procedures.