Disruption of the gene XRN1, coding for a 5′→3′ exoribonuclease, restricts yeast cell growth

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

W. Neal Burnette · Analytical Biochemistry · 1981 · 8.5K citations

Biochemistry, Natural Sciences, Bioanalysis +10

Transformation of intact yeast cells treated with alkali cations

Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983 · 7.7K citations · Full text

Cell Division, Natural Sciences, Biotechnology +15

[12] One-step gene disruption in yeast

Rodney Rothstein · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 3.3K citations

Genetics, Yeast, Molecular Genetics +2

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

Marian Carlson, David Botstein · Cell · 1982 · 1.5K citations

Intracellular Forms, Transcriptional Regulation, Different 5′ +13