Chromosomal Disturbances Observed in Human Fetal Renal Cells Transformed <i>in vitro</i> by Simian Virus 40 and Carried in Culture

Abstract

Karyological disturbances observed during the 6th to 17th subcultures in three lines of SV<sub>40</sub>-transformed human fetal renal cells were not evident in the 1st to 3rd subcultures of uninoculated control cells. The available evidence supports the hypothesis that the observed aberrations reflect changes in the cell genome attributable to cell-virus interaction at the time of transformation. An ancillary role of environmental factors during subcultivation subsequent to transformation in the selection of certain of the observed abnormalities is suggested by the experimental findings.