Publication | Open Access

Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensis

Natural SciencesGeneticsVirulence FactorMolecular BiologyGenetic EngineeringDna ReplicationUnstable PlasmidMolecular GeneticsMicrobiologyMolecular MicrobiologyI-scei EndonucleaseMedicineFrancisella TularensisGene Deletion DataGenome EditingMutagenesis