Multiple replacements establish the importance of tyrosine-503 in β-galactosidase (Escherichia coli)

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

Mark J. Zoller, Michael J. Smith · DNA · 1984 · 906 citations

Life Sciences, Genetics, Molecular Biology +15

Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli

Barbara Kramer, Wilfried Kramer, Hans‐Joachim Fritz · Cell · 1984 · 466 citations

Life Sciences, Genetics, Molecular Biology +13

Sequence of the lacZ gene of Escherichia coli.

A. Kalnins, Karin E Otto, Ulrich Rüther et al. · The EMBO Journal · 1983 · 455 citations · Full text

Life Sciences, Genetics, Microbiology +6

On the source of intramolecular and enzymatic reactivity

Fredric M. Menger · Accounts of Chemical Research · 1985 · 268 citations

Life Sciences, Biochemistry, Biocatalysis +5