Concepedia

Publication | Closed Access

Design and Anti-HIV-1 Activity of Hammerhead and Hairpin Ribozymes Containing a Stable Loop

DOI

11

Citations

0

References

1998

Year

Makoto Koizumi, Yuji Ozawa, Ryuichi Yagi, Takashi Nishigaki, Masakatsu Kaneko, Shinichi Oka, Satoshi Kimura, Aikichi Iwamoto, Yasuo Komatsu, Eiko Ohtsuka
Nucleosides Nucleotides & Nucleic Acids

Abstract

Abstract Three ribozymes, a hairpin ribozyme (HR112) and two hammerhead ribozymes (RZ115 and RZ119) containing a 5′C(UUCG)G3′ loop were designed to cleave the U5 region in the long terminal repeat (LTR) of HIV-1 RNA. The t½ values of chemically synthesized substrates mediated by three ribozymes were measured. The transformed CEM cells possessing these three ribozyme-encoding genes were challenged with a HIV-1IIIB strain, and two of these three ribozymes, HR112 and RZ119, were shown to possess strong anti-HIV-1 activity.