Plant Regeneration from Protoplasts in <i>Linum</i>*

Abstract

Abstract Protoplasts of eight wild Linum species and 14 genotypes of flax, isolated from different parts of seedlings and from shoot apices, were regenerated to callus in medium V‐KM (B inding and N ehls 1977). Plant regeneration was obtained in L. alpinum, L. amurense, L. hologynum, L. perenne, L. salsoloides and eight genotypes of L. usitatissimum on agar media B5 (G amborg et al. 1968), B5C (B inding et al, 1981), MS (M urashige and S koog 1962) and MSC (MS with 5% coconut endosperm) containing 2.5μM 6‐BA. An adventitious embryo was regenerated in L. alpinum. Callus differentiation like sponge parenchyma, was observed in protoplast‐derived tissues of L. usitatissimum. Shoots organized roots under slightly divergent conditions in the different species.