Linear electrical properties of striated muscle fibres observed with intracellular electrodes

Abstract

Abstract The linear electrical properties of muscle fibres have been examined using intracellular electrodes for a. c. measurements and analyzing observations on the basis of cable theory. The measurements have covered the frequency range 1 c/s to 10 kc/s. Comparison of the theory for the circular cylindrical fibre with that for the ideal, one-dimensional cable indicates that, under the conditions of the experiments, no serious error would be introduced in the analysis by the geometrical idealization. The impedance locus for frog sartorius and crayfish limb muscle fibres deviates over a wide range of frequencies from that expected for a simple model in which the current path between the inside and the outside of the fibre consists only of a resistance and a capacitance in parallel. A good fit of the experimental results on frog fibres is obtained if the inside-outside admittance is considered to contain, in addition to the parallel elements Rm = 3100 Ωcm2 and Cm = 2.6 μF/cm2, another path composed of a resistance Re = 330 Ωcm2 in series with a capacitance Ce = 4.1 μF/cm2, all referred to unit area of fibre surface. The impedance behaviour of crayfish fibres can be described by a similar model, the corresponding values being Rm = 680 Ωcm2, Cm = 3.9 μF/cm2, Re = 35 Ωcm2, Ce = 17 μF/cm2. The response of frog fibres to a step-function current (with the points of voltage recording and current application close together) has been analyzed in terms of the above two-time constant model, and it is shown that neglecting the series resistance would have an appreciable effect on the agreement between theory and experiment only at times less than the halftime of rise of the response. The elements Rm and Cm are presumed to represent properties of the surface membrane of the fibre. Re and Ce are thought to arise not at the surface, but to be indicative of a separate current path from the myoplasm through an intracellular system of channels to the exterior. In the case of crayfish fibres, it is possible that Re (when referred to unit volume) would be a measure of the resistivity of the interior of the channels, and Ce the capacitance across the walls of the channels. In the case of frog fibres, it is suggested that the elements Re, Ce arise from the properties of adjacent membranes of the triads in the sarcoplasmic reticulum . The possibility is considered that the potential difference across the capacitance Ce may control the initiation of contraction.