Bausteine von Oligosacchariden, CI. Synthese von modifizierten Oligosacchariden der <i>N</i>‐Glycoproteine zur Untersuchung der Substratspezifität der <i>N</i>‐Acetylglucosaminyltransferase II des Trimming‐Prozesses

Abstract

Building Units of Oligosaccharides, CI. — Synthesis of Modified Oligosaccharides of the N ‐Glycoproteins Intended for Substrate Specificity Studies of N ‐Acetylglucosaminyltransferase II of the Trimming Process The trichloroacetimidate method was very successful for the synthesis of the tetrasaccharide octyl O ‐(2‐acetamido‐2‐deoxy‐β‐ D ‐glucopyranosyl)‐(1→2)‐ O ‐(α‐ D ‐mannopyranosyl)‐(1→3)‐ O ‐[(α‐ D ‐mannopyranosyl)‐(1→6)]‐β‐ D ‐mannopyranoside ( 10 ). Further derivatives of the tetrasaccharide with a 4‐ O ‐methyl group 20 and with a 4‐deoxy group 28 were synthesized. The tetrasaccharides are intended for substrate specificity studies of the N ‐acetylglucosaminyltransferase II of the trimming process in the biosynthesis of N ‐glycoproteins.